BERKELEY SENSOR & ACTUATOR CENTER
UC BERKELEY UC DAVIS
User: Guest |  Site Map |  My BSAC Profile
HOME  PROJECTS  THRUSTS  PUBLICATIONS  ABOUT BSAC  DIRECTORY  ALUMNI  FOR BSAC RESEARCHERS  EVENTS CALENDAR  SECURE LOGIN
Table of all Projects
     
 

BPN831: Sample-to-Answer Multiplex Molecular Diagnostics for Subtyping Pathogenic Bacteria

Project ID BPN831
Website
Start Date Wed 2016-Feb-10 16:38:44
Last Updated Tue 2016-Mar-01 19:48:23
Abstract The rapid and sensitive detection of pathogenic bacteria is crucial for identifying the pathogen and patient care with precise antibiotic treatment in public health. Thus, various diagnostic approaches such as a sequencing, enzyme-linked immunosorbent assay (ELISA), antibiotic susceptibility test (AST) have been used, but current methods still lack for practical application due to long processing time, and a large equipment- based operation. Here, we report a macrofluidic diagnostic platform, capable of rapid sample preparation, pathogen lysis and genetic profiling of multiple pathogens directly in clinical urine samples within 20 minutes. Membrane-based, absorbent pad-assisted bacteria preconcentrator demonstrates “urine sample in - bacteria out” capability, achieving 90% recovery efficiency. The photothermal lysis and polymerase chain reaction (PCR)-based nucleic acid amplification allow specific detection of multiple pathogens for subtyping of pathogens such as K. pneumonia, P. aeruginosa, E. coli and C. freundii. This integrated diagnostic platform described here enables a rapid diagnosis and identification of clinically relevant bacterial species for targeted therapeutic intervention and precision medicine.
Status New
Funding Source Industry
IAB Research Area BioMEMS
Researcher(s) Sang Hun Lee, Byungrae Cho, Jun Ho Son
Advisor(s) Luke P. Lee
Detailed Information
Secure Access

Private Abstract
Research Report
Poster
Summary Slide PDF | VIDEO
Active Feedback (or Request for Response)

 

  • Copyright Notification: All papers downloaded from this site are © University of California or the publisher, all rights reserved. Contact the BSAC Webmaster for permission related to copyrighted materials.
  • Links on these pages to commercial sites do not represent endorsements by UC or its affiliates.
  • Privacy Policy
  • Contact Us

   webmaster@bsac.eecs.berkeley.edu
  User logged in as: Guest
  User Idle since: August 17, 2017, 7:55 pm